Plasma Proteins Assays

Plasma Proteins

Kit Description Catalogue Number
Glycaben (h Gly Albumin ELISA, Strip Plate) 1003
Glycabumin 50 (r Gly Albumin ELISA, Tubes) GAR-50
Glyco-Albumin R (r Gly Albumin ELISA, Strip Plate) 1017
Apo B (h Apolipoprotein B ELISA, Strip Plate) 1006
Glycacor (h Gly ApoB ELISA, Strip Plate) 1009
Fibronectin ELISA (h Fibronectin ELISA, Strip Plate) 1010
CRP ELISA (m/r C-reactive protein ELISA, Strip Plate) 1022

h = human; m = mouse; r = rat: c = canine; p = primate, non human

A brief summary of the assays is available by selecting the relevant tab below.

Glycaben
Glycaben:
Catalogue Numbers: 1003 Strip Plate
Methodology: Sandwich ELISA
Summary of procedure: Glycaben is an enzyme linked immunosorbent assay for the quantitative determination of glycoalbumin in human plasma. It provides information concerning glycemic control analogous to that offered by glycohemoglobin measurements, except that the protein measured is glycated albumin which has a shorter half-life.

The assay depends upon a monoclonal antibody, A717, that specifically recognizes the glycated moieties in glycated albumin. In practice, standards or samples are added to A717 coated plates (Glycaben). The glycoalbumin in these analytes binds to or is captured by the antibody on the solid phase, and unbound materials are washed away. A subsequent incubation with an enzyme conjugated antihuman albumin antibody leads to detection of the bound molecules. Bound conjugate is determined colorimetrically after reaction with a TMB substrate solution.

By relating sample response with that for the standards, the concentration of glycoalbumin may be determined. A separate analysis of total albumin allows the results to be converted to percent of total albumin that is glycated.

Specimen Required: 50ul citrate or EDTA treated plasma
Assay Range: 0.1-3 mg/ml glycated albumin
Sensitivity: 0.1 mg/ml
Precision: Intra- and interassay precision of samples within the assay range have a C.V. <10%.
Specificity: The monoclonal antibody A717 is specific for glycated albumin, and does not cross-react with other proteins that occur in human plasma including non-glycated albumin. The enzyme conjugated antibody preparation is specific for human albumin.
Expected Values: Plasma from normal non-diabetic individual have 0.4-2.4% glycated albumin whereas that from uncontrolled diabetics will be >2.5%.
Glycabumin 50
Glycabumin :  
Catalogue Numbers: GAR-50
50 determination tube assay
Methodology: Sandwhich ELISA
Summary of procedure: Glycabumin is an enzyme linked immunosorbent assay for the quantitative determination of glycoalbumin in rat plasma. It provides information concerning glycemic control analogous to that offered by glycohemoglobin measurements, except that the protein measured is glycated albumin which has a shorter half-life.

The assay depends upon a monoclonal antibody, A717, that specifically recognizes the glycated moieties in glycated albumin. In practice, standards or samples are added to A717coated tubes. The r glycoalbumin in these analytes binds to or is captured by the antibody on the solid phase, and unbound materials are washed away. A subsequent incubation with an enzyme conjugated anti-rat albumin antibody leads to detection of the bound molecules. Bound conjugate is determined colorimetrically after reaction with a TMB substrate solution.

By relating samples response with that for the standard, the concentration of rat glycoalbumin may be determined. A separate analysis of total albumin allows the results to be converted to percent total albumin that is glycated.

Specimen Required: 50ul citrate of EDTA treated plasma
Assay Range: 0.2-2 mg/ml glycated albumin
Precision: Intra- and interassay precision of samples within the assay range have a C.V. <10%.
Specificity: The monoclonal antibody A717 is specific for glycated albumin, and does not cross-react with other proteins that occur in human plasma including non-glycated albumin. The enzyme conjugated antibody preparation is specific for rat albumin.
Glyco-Albumin R
Manufacturer/Trade: Exocell / Glyco-Albumin R
Catalogue Numbers: 1017 Strip Plate
Methodology: Sandwich ELISA
Summary of procedure:

The Glyco-Albumin R assay is an ELISA designed specifically for use with rat serum samples. In practice, sera are diluted 1:11 to fall within the useful range of the assay.

The assay depends upon the binding of rat glycated albumin in standard or sample to specially treated microtiter wells. Bound analyte is detected with an anti-rat albumin antibody-HRP conjugate. After a final wash, bound conjugate is detected using a chromogenic substrate (TMB) for peroxidase, and color intensity is directly porportional to glycated albumin concentration. Typical glycated albumin values lie between 0.1 and 0.6 mg/dL, abut normal values may vary with different stranis of rats.

Specimen Required: Serum: 20 uL
Assay Range: 0.1-0.6 mg/mL
Precision: Intraassay and interassay precision for samples within the useful range of the assay have a C.V.<10% of the mean.
Apo B
Apo B Test :  
Catalogue Numbers: 1006
Methodology: Indirect Competitive ELISA
Summary of procedure: The Apo B Test is designed for the quantitative measurement of apolipoprotein B in human plasma. To complete the assay, B36 monoclonal antibody is added to a microtiter plate well that contains apolipoprotein B immobilized to the solid phase, and to which apolipoprotein B, standard or sample, is added in the fluid phase. During a 30 minute incubation, the monoclonal antibody binds to either the immobilized apo B or to the soluble apo B, hence the notion of competitive binding. Unbound reactants are then washed away, and a second incubation of 15 minutes with an enzyme conjugated to an anti-mouse antibody reacts with the B36 that was captured by the solid phase. The wells are washed to remove unbound secondary antibody, and TMB Substrate solution is added to detect bound conjugate. The chromogenic reaction is stopped by an addition of dilute acid, and color intensity determined by absorbance at 450nm. Sample concentration is determined by relating response to that for known standards.
Specimen Required: 10ul citrate or EDTA treated plasma
Assay Range: 0.13-2.0 mg/ml (corresponding to 13-200mg/dl)
Precision: Intra- and interassay precision of samples within the assay range have a C.V.<10%.
Specificity: The monoclonal antibody B36 is specific for human apolipoprotein B, and does not cross-react with other proteins or substances found in human plasma.
Expected Values: Expected values for apo B in male subjects are reported to be 108 +/- 33 mg/dl for individuals free of coronary artery disease (CAD), and 131 +/- 37 for subjects with angiographically documented premature CAD. Values for women are similar to those for men.
Glycacor
Glycacor :  
Catalogue Numbers: 1009 Strip Plate
Methodology: Indirect Competitive ELISA
Summary of procedure: Non-radioactive immunoassay for the quantitative determination of glycated LDL in plasma to evaluate glycemic control in diabetes, and to assess the contribution of the modified protein to vascular disease. The assay depends upon ES12, a monoclonal antibody directed against a specific epitope in glycated LDL. The glycated LDL is measured in apolipoprotein B equivalents, and is expressed as ug/ml or mg/dl. Glycacor is a competitive ELISA in a microplate format.

To complete the assay, the ES12 is added to a microtiter plate that contains immobilized glycated LDL and soluble glycated LDL (standard or experimental sample). The monoclonal antibody binds to either the immobilized glycated LDL or the soluble phase glycated LDL during the primary incubation, hence the notion of competitive binding. Reactants in the fluid phase are subsequently washed away, and captured ES12 on the solid phase is detected with an enzyme conjugated anti-mouse antibody. The results are made quantitative after chromogenic reaction with TMB.

Specimen Required: 20ul Citrate or EDTA treated plasma
Assay Range: 3-40ug/ml (corresponding to 0.3 - 4mg/dl)
Precision: Intra and interassay precision of samples within the useful range of the assay have a C.V.<10%.
Specificity: ES12 is specific for glycated LDL, and does not cross-react with other human plasma proteins including non-glycated LDL in either ELISA or Western Blot formats.
Fibronectin ELISA
h Fibronectin ELISA : Exocell /
Catalogue Numbers: 1010 Strip Plate
Methodology: Sandwich ELISA
Summary of procedure: The Fibronectin ELISA is a sandwich assay for the quantitative measurement of fibronectin in freshly drawn human plasma. The assay depends upon the binding of fibronectin, in standard or in plasma, to specially coated microtiter wells. The plate is washed to remove unbound reactants, and fibronectin is detected with an anti-fibronectin antibody-HRP conjugate. The plate is again washed, and TMB is added to detect bound HRP. The chromogenic reaction is stopped with dilute sulfuric acid, and color intensity determined using a microplate reader stet at 450nm. Absorbance is directly proportional to the logarithm of fibronectin concentration added in the sample. The assay may be completed in less than 3 hours.
Specimen Required: Fresh plasma (citrate or EDTA), 10ul
Assay Range: 0.2-5.0 mg/dl
Precision: Intraassay and interassay precision for samples within the useful range have a C.V.<10%.
Specificity: h Fibronectin shows some cross-reactivity with murine plasma fibronectin.
Expected Values: 20-40mg/dl